Pathogens transmitting between the environment, wildlife, livestock and humans are major health concerns for human and domestic animal and in addition, for the sustainability of agriculture and the conservation of wildlife. Among pathogens causing zoonosis, Genus Mycobacterium including Mycobacterium tuberculosis, M. bovis, M. avium is thought to be important. The most important bacteria as an etiological agent of zoonosis in Genus Mycobacterium is M. bovis. M. bovis is the causative agent of bovine tuberculosis, which belongs to the M. tuberculosis complex and has a broad host range including wildlife, domestic livestock, non-human primates and humans. The reservoirs of M. bovis in wildlife have their own role as sources of infection in humans and domestic animals and have their health impact on humans. The approaches for the control and management of M. bovis infections are also discussed in this review.