Mycobacterium tuberculosis complex differentiation by genomic deletion patterns with multiplex polymerase chain reaction and melting analysis

June I Pounder; Clint M Anderson; Karl V Voelkerding; Max Salfinger; Jillian Dormandy; Akos Somoskovi; Leonid Heifets; Jenny Jo Graham; Douglas R Storts; Cathy A Petti

doi:10.1016/j.diagmicrobio.2009.12.014

Mycobacterium tuberculosis complex differentiation by genomic deletion patterns with multiplex polymerase chain reaction and melting analysis

Diagn Microbiol Infect Dis. 2010 May;67(1):101-5. doi: 10.1016/j.diagmicrobio.2009.12.014. Epub 2010 Mar 12.

Authors

June I Pounder¹, Clint M Anderson, Karl V Voelkerding, Max Salfinger, Jillian Dormandy, Akos Somoskovi, Leonid Heifets, Jenny Jo Graham, Douglas R Storts, Cathy A Petti

Affiliation

¹ Institute for Clinical and Experimental Pathology, Associated Regional and University Pathologists, Inc., Salt Lake City, UT 84108, USA. afjip@uaa.alaska.edu

PMID: 20227227
DOI: 10.1016/j.diagmicrobio.2009.12.014

Abstract

Differentiation of Mycobacterium tuberculosis complex (MTC) species is important for patient management. We developed a genomic deletion assay based on multiplex polymerase chain reaction with melting temperature analysis that correctly identified 124 (96%) of 129 MTC isolates. This assay is a fast single-tube method to differentiate members of MTC.

Publication types

Comparative Study
Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Bacteriological Techniques / methods*
DNA, Bacterial / genetics*
Humans
Mycobacterium tuberculosis / classification
Mycobacterium tuberculosis / genetics
Mycobacterium tuberculosis / isolation & purification*
Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Sequence Deletion*
Transition Temperature
Tuberculosis / microbiology*

Substances

DNA, Bacterial