Production of human growth hormone by Lactococcus lactis

Abelardo Margolles; José Antonio Moreno; Lorena Ruiz; Belkis Marelli; Christian Magni; Clara G de Los Reyes-Gavilán; Patricia Ruas-Madiedo

doi:10.1016/j.jbiosc.2009.10.006

Production of human growth hormone by Lactococcus lactis

J Biosci Bioeng. 2010 Apr;109(4):322-4. doi: 10.1016/j.jbiosc.2009.10.006. Epub 2009 Oct 27.

Authors

Abelardo Margolles¹, José Antonio Moreno, Lorena Ruiz, Belkis Marelli, Christian Magni, Clara G de Los Reyes-Gavilán, Patricia Ruas-Madiedo

Affiliation

¹ Departamento de Microbiología y Bioquímica de Productos Lácteos, Instituto de Productos Lácteos de Asturias (CSIC), Carretera de Infiesto s/n 3300 Villaviciosa, Asturias, Spain. amargolles@ipla.csic.es

PMID: 20226370
DOI: 10.1016/j.jbiosc.2009.10.006

Abstract

A synthetic gene coding for human growth hormone was expressed in Lactococcus lactis. The presence of the recombinant protein was assayed and quantified using ELISA tests. Human growth hormone was detected at high concentrations and displayed a biological activity similar to the one shown by commercial human growth hormone.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Caco-2 Cells
Cell Proliferation / drug effects
DNA Primers / genetics
Genetic Engineering / methods
Genetic Vectors
Human Growth Hormone / biosynthesis*
Human Growth Hormone / genetics*
Human Growth Hormone / pharmacology
Humans
In Vitro Techniques
Lactococcus lactis / genetics*
Lactococcus lactis / metabolism*
Recombinant Proteins / biosynthesis
Recombinant Proteins / genetics
Recombinant Proteins / pharmacology

Substances

DNA Primers
Recombinant Proteins
Human Growth Hormone