Abstract
A novel, easy to perform PCR-based method employing specific DNA methylation biomarkers to detect B-cell neoplasms in a variety of B-cell lines and B lymphoblastic leukemia (B-ALL) patient specimens has been developed. This method detects as few as 5 B-ALL cells, or 1 B-ALL cell in 1,000,000 normal background blood cells using a single marker, DLC-1 gene CpG island (CGI) methylation. By adding two additional markers PCDHGA12 and RPIB9, over 80% of B-ALL cases were detected in patients' bone marrow and/or peripheral blood specimens. We have traced clinical B-ALL cases up to 10 years retrospectively and the DLC-1 methylation is correlated with patient clinical status. Thus, this epigenetic-based molecular method demonstrates its potential use in the diagnosis of B-cell neoplasia, in addition to traditional approach such as clinical features, morphology, immunophenotype, and genetic analysis.
Keywords:
B lymphoblastic leukemia; DNA methylation biomarker; Mature B-cell neoplasms.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adolescent
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Adult
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Aged
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Aged, 80 and over
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Biomarkers, Tumor / genetics
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Biomarkers, Tumor / metabolism*
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Carrier Proteins / genetics
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Carrier Proteins / metabolism
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Cell Line, Tumor
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Child
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Child, Preschool
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DNA Methylation*
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DNA, Neoplasm / genetics*
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Female
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GTPase-Activating Proteins
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Humans
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Intracellular Signaling Peptides and Proteins
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Leukemia, B-Cell / diagnosis
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Leukemia, B-Cell / genetics
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Leukemia, B-Cell / metabolism*
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Lymphoma, B-Cell / diagnosis
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Lymphoma, B-Cell / genetics
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Lymphoma, B-Cell / metabolism*
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Male
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Middle Aged
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / metabolism
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Retrospective Studies
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Sensitivity and Specificity
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Tumor Suppressor Proteins / genetics
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Tumor Suppressor Proteins / metabolism
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Young Adult
Substances
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Biomarkers, Tumor
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Carrier Proteins
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DLC1 protein, human
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DNA, Neoplasm
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GTPase-Activating Proteins
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Intracellular Signaling Peptides and Proteins
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Nerve Tissue Proteins
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RUNDC3B protein, human
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Tumor Suppressor Proteins