Peptide 68-88 of apocytochrome c plays a crucial role in its insertion into membrane and binding to mitochondria

Xiaoping Wang; Xuehai Han; Fuyu Yang

doi:10.1007/BF03182681

Peptide 68-88 of apocytochrome c plays a crucial role in its insertion into membrane and binding to mitochondria

Sci China C Life Sci. 2003 Feb;46(1):18-27. doi: 10.1007/BF03182681.

Authors

Xiaoping Wang¹, Xuehai Han, Fuyu Yang

Affiliation

¹ National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.

PMID: 20213358
DOI: 10.1007/BF03182681

Abstract

Apocytochrome c (Apocyt. c) is the precursor of cytochrome c. It is synthesized in the cytosol and posttranslationally imported into mitochondria. In order to determine the crucial sequence in apocyt. c translocation, deleted mutant and chemically synthesized peptides with different length were used. Obtained results showed that sequence 68-88 of apocyt. c plays a critical role in its insertion into membrane and binding to mitochondria.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Avian Proteins / chemistry
Avian Proteins / genetics
Avian Proteins / metabolism
Chickens
Cytochromes c / chemistry*
Cytochromes c / genetics
Cytochromes c / metabolism*
In Vitro Techniques
Membrane Lipids / metabolism
Mitochondrial Membranes / metabolism*
Molecular Sequence Data
Mutagenesis, Site-Directed
Peptide Fragments / chemistry
Peptide Fragments / genetics
Peptide Fragments / metabolism
Recombinant Proteins / chemistry
Recombinant Proteins / genetics
Recombinant Proteins / metabolism
Sequence Deletion

Substances

Avian Proteins
Membrane Lipids
Peptide Fragments
Recombinant Proteins
Cytochromes c