Abstract
The aim of this study was to investigate the preventive effect of Agrocybe chaxingu polysaccharide on streptozocin (STZ)-induced pancreatic beta-cells destruction. Agrocybe chaxingu polysaccharide markedly reduced nitric oxide (NO) production and iNOS expression levels in RINm5F cells in a dose-dependent manner. In addition, Agrocybe chaxingu polysaccharide significantly inhibited iNOS expression and blood glucose levels in STZ-induced diabetic mice. Moreover, immunohistochemical analysis revealed that it enhanced pancreatic beta-cells resistance to destruction by STZ. These results suggest that Agrocybe chaxingu polysaccharide may have value as a therapeutic agent against diabetes mellitus.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Agrocybe / chemistry*
-
Animals
-
Blood Glucose / metabolism
-
Blotting, Western
-
Carbohydrate Sequence
-
Cell Line, Tumor
-
DNA Fragmentation / drug effects
-
Diabetes Mellitus, Experimental / chemically induced
-
Diabetes Mellitus, Experimental / metabolism
-
Diabetes Mellitus, Experimental / prevention & control*
-
Dose-Response Relationship, Drug
-
Electrophoresis, Agar Gel
-
Insulin-Secreting Cells / drug effects
-
Insulin-Secreting Cells / metabolism
-
Male
-
Mice
-
Mice, Inbred ICR
-
Molecular Sequence Data
-
Nitric Oxide / metabolism
-
Nitric Oxide Donors / pharmacology
-
Nitric Oxide Synthase Type II / genetics
-
Nitric Oxide Synthase Type II / metabolism
-
Nitroprusside / pharmacology
-
Polysaccharides / chemistry
-
Polysaccharides / pharmacology*
-
Reverse Transcriptase Polymerase Chain Reaction
-
Streptozocin
Substances
-
Blood Glucose
-
Nitric Oxide Donors
-
Polysaccharides
-
Nitroprusside
-
Nitric Oxide
-
Streptozocin
-
Nitric Oxide Synthase Type II