Immune response to intranasal and intraperitoneal immunization with Kaposi's sarcoma-associated herpesvirus in mice

Vaccine. 2010 Apr 26;28(19):3325-32. doi: 10.1016/j.vaccine.2010.02.091. Epub 2010 Mar 4.

Abstract

A vaccine for Kaposi's sarcoma-associated herpesvirus (KSHV) is not currently available. To obtain the fundamental data in animals for vaccine development, KSHV particles were immunized to Balb/c mice through intraperitoneal and intranasal routes in the present study. Intranasal immunization with KSHV induced IgA to KSHV in not only serum, but also nasal wash fluid and saliva. A neutralization assay using recombinant KSHV that expressed green fluorescent protein revealed that nasal wash fluid and saliva from the KSHV-immunized mice neutralized KSHV infection to human embryonic kidney 293 cells in vitro in a dose-dependent manner to KSHV copies immunized. The serum and nasal wash fluid of KSHV-encoded K8.1 protein-immunized mice neutralized KSHV infection to 293 cells in vitro. These data suggest a possibility of mucosal vaccine for prophylaxis of KSHV infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Intranasal
  • Animals
  • Antibodies, Neutralizing / analysis
  • Cell Line
  • Female
  • Herpesvirus 8, Human / immunology*
  • Herpesvirus Vaccines / administration & dosage
  • Herpesvirus Vaccines / immunology*
  • Humans
  • Immunoglobulin A / analysis
  • Immunoglobulin A / blood
  • Injections, Intraperitoneal
  • Mice
  • Mice, Inbred BALB C
  • Nasal Mucosa / immunology
  • Saliva / immunology
  • Serum / immunology

Substances

  • Antibodies, Neutralizing
  • Herpesvirus Vaccines
  • Immunoglobulin A