Abstract
The KY protein underlies a form of muscular dystrophy in the mouse but its role in muscle remains elusive. Immunodetection of endogenous KY protein in C2C12-derived myotubes and expression of a recombinant form in neonatal cardiomyocytes indicated that KY is a Z-band associated protein. Moreover, characterization of a KY interacting protein fragment led to the identification of Igfn1 (Immunoglobulin-like and fibronectin type 3 domain containing 1). Igfn1 is a transcriptionally complex locus encoding many protein variants. A yeast two-hybrid screen identified the Z-band protein filamin C (FLNC) as an interacting partner. Consistent with this, expression of an IGFN1 recombinant fragment showed that the three N-terminal globular domains, common to at least five IGFN1 variants, are sufficient to provide Z-band targeting. Taken together, the yeast two-hybrid, biochemical and immunofluorescence data support the notion that KY, IGFN1 and FLNC are part of a Z-band associated protein complex likely to provide structural support to the skeletal muscle sarcomere.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Carrier Proteins / chemistry*
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Carrier Proteins / genetics
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Carrier Proteins / isolation & purification
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Cell Line
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Contractile Proteins / chemistry*
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Contractile Proteins / genetics
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Contractile Proteins / isolation & purification
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DNA Primers / genetics
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DNA, Complementary / genetics
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DNA, Complementary / isolation & purification
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Filamins
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In Vitro Techniques
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Mice
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Microfilament Proteins / chemistry*
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Microfilament Proteins / genetics
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Microfilament Proteins / isolation & purification
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Multiprotein Complexes / chemistry
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Multiprotein Complexes / isolation & purification
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Muscle Fibers, Skeletal / chemistry
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Muscle Proteins / chemistry*
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Muscle Proteins / genetics
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Muscle Proteins / isolation & purification
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Muscle, Skeletal / chemistry
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Myocytes, Cardiac / metabolism
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Peptide Hydrolases
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Protein Interaction Mapping
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Recombinant Proteins / isolation & purification
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Sarcomeres / chemistry
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Tissue Distribution
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Two-Hybrid System Techniques
Substances
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Carrier Proteins
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Contractile Proteins
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DNA Primers
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DNA, Complementary
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Filamins
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Microfilament Proteins
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Multiprotein Complexes
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Muscle Proteins
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Recombinant Proteins
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KY protein, mouse
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Peptide Hydrolases