Multicellular aggregates (spheroids) represent an intermittent level between monolayer growing cells and tissue culture. Spheroids are rather objective model of the three-dimensional growth and organization, the cell-to-cell interactions and influence of microenvironmental conditions on tumour microaggregates. In our work formation and growth of spheroids depends on concentration of CMC and FCS. Conditions of microenvironment influence on intensiveness of proliferation as well as on cells adhesiveness and formation of microaggregates.