The aim of this study was evaluation of influence of culture conditions (nutrient availability, pH, temperature) on adhesion E. coli strains to polystyrene. This study included 74 of E. coli strains isolated from urine and blood samples. All of these strains were isolated in the Clinical Microbiology Department of Dr. A. Jurasz University Hospital in 2003-2006. Analyzed strains most often adhered to polystyrene after incubation in high-nutrient medium, at pH values between 5.0 and 7.0, at 37 degrees C.