In vitro selection of RNA aptamers to a small molecule target

Curr Protoc Nucleic Acid Chem. 2010 Mar:Chapter 9:Unit 9.5.1-23. doi: 10.1002/0471142700.nc0905s40.

Abstract

This unit describes the selection of aptamers from a single-stranded RNA pool that bind to small molecule targets. Aptamers generated by this type of selection experiment can potentially function as receptors for small molecules in numerous applications, including medical diagnostics, therapeutics, and environmental monitoring. This unit describes two modes of selection, one by column filtration and one by batch selection.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Aptamers, Nucleotide / chemistry
  • Aptamers, Nucleotide / genetics
  • Aptamers, Nucleotide / metabolism*
  • DNA, Single-Stranded / chemistry
  • DNA, Single-Stranded / genetics
  • DNA, Single-Stranded / metabolism
  • Filtration
  • Oligonucleotides / chemistry
  • Oligonucleotides / genetics
  • Oligonucleotides / metabolism*
  • RNA / chemistry
  • RNA / genetics
  • RNA / metabolism*

Substances

  • Aptamers, Nucleotide
  • DNA, Single-Stranded
  • Oligonucleotides
  • RNA