[Effects of silencing chloride intracellular channel 1 gene expression on the proliferation and invasion of mouse hepatocellular carcinoma cell lines]

Zhonghua Gan Zang Bing Za Zhi. 2010 Feb;18(2):131-5. doi: 10.3760/cma.j.issn.1007-3418.2010.02.012.
[Article in Chinese]

Abstract

Objective: To study the effects of silencing CLIC1 gene expression on the proliferation and invasion of Hca-F cells.

Methods: The mouse CLIC1 cDNA sequence was retrieved from NCBI. Three shRNA sequences were designed and cloned into pGPU6/GFP/Neo plasmids. The plasmids were transfected into Hca-F cells with Lipofectamine 2000. Cell Counting-8 (CCK-8) kit and transwell chamber were used to study the effects of CLIC1 on the proliferation and invasion of Hca-F cells.

Results: The pGPU6/GFP/Neo-shRNA-3 plasmid effectively repressed the expression of CLIC1 mRNA. Inhibition of CLIC1 gene expression led to decreased cell proliferation and reduced invasion.

Conclusion: CLIC1 is essential for the proliferation and invasion of Hca-F cells.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carcinoma, Hepatocellular / metabolism
  • Carcinoma, Hepatocellular / pathology*
  • Cell Line, Tumor
  • Cell Proliferation*
  • Chloride Channels / antagonists & inhibitors
  • Chloride Channels / genetics*
  • Chloride Channels / metabolism
  • Gene Expression Regulation, Neoplastic
  • Genetic Vectors
  • Liver Neoplasms / metabolism
  • Liver Neoplasms / pathology*
  • Mice
  • Neoplasm Invasiveness
  • Plasmids / genetics
  • RNA Interference*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • RNA, Small Interfering / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transfection

Substances

  • Chloride Channels
  • Clic1 protein, mouse
  • RNA, Messenger
  • RNA, Small Interfering