Objective: To investigate the effects of high mobility group box-1 (HMGB1) silencing upon the invasion and proliferation in human lung cancer cell L9981 by RNA inhibition.
Methods: L9981 cells were divided into 3 groups. Group 1 was transfected by HMGB1 small interfering RNA (HMGB1-siRNA group). Group 2 was transfected by negative sequence small interfering RNA (negative control group). Group 3 was blank group. The mRNA and protein of HMGB1 were determined by real-time PCR and Western blotting assay respectively. The proliferation ability was examined by cell viability assay. The growth status of cells was examined by MTT at 24, 48, 72 and 96 h post-transfection. Invasion ability was evaluated by Boyden chamber model.
Results: The relative expression of HMGB1 mRNA of HMGB1-siRNA group (1.0 +/- 0.0) was much lower than the negative control (12.8 +/- 1.3, P < 0.05) and blank groups (12.1 +/- 1.0, P < 0.05). HMGB1 protein expression was also significantly inhibited. Cell viability of HMGB1-siRNA group was much lower than other two groups. MTT indicated the growth of HMGB1-siRNA group was significantly inhibited than other two groups. Boyden chamber model indicated the number of penetrating membrane in HMGB1-siRNA group was less than other two groups.
Conclusion: Down-regulating HMGB1 gene expression by HMGB1 siRNA can inhibit the invasion and proliferation of human lung cancer cell.