Percentage of regulatory T cells CD4+CD25+CD127- in HIV-infected patients is not reduced after cryopreservation

Fabienne Venet; Christophe Malcus; Tristan Ferry; Françoise Poitevin; Guillaume Monneret

doi:10.1016/j.jim.2010.02.005

Percentage of regulatory T cells CD4+CD25+CD127- in HIV-infected patients is not reduced after cryopreservation

J Immunol Methods. 2010 May 31;357(1-2):55-8. doi: 10.1016/j.jim.2010.02.005. Epub 2010 Feb 25.

Authors

Fabienne Venet¹, Christophe Malcus, Tristan Ferry, Françoise Poitevin, Guillaume Monneret

Affiliation

¹ Hospices Civils de Lyon, Hôpital E. Herriot, Pavillon E, 5 place d'Arsonval, 69437 LYON Cedex 03, France.

PMID: 20188734
DOI: 10.1016/j.jim.2010.02.005

Abstract

Interest in regulatory T cells (Treg) has been continuously growing over the last 10years. In particular, a role for Treg has been recently reported in HIV. It is crucial to investigate their conservation over time, once sampled and purified. The objective of the present study was to investigate whether cryopreservation may modify recognition and measurement of relative frequency of Treg (CD4(+)CD25(+)CD127(-)) among PBMCs purified from 17 HIV-infected patients. Ours results indicate that cryopreservation does not affect this specific T lymphocyte subset.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CD4 Antigens / blood
CD4 Antigens / immunology
CD4 Lymphocyte Count
Cohort Studies
Cryopreservation*
Female
HIV Infections / blood*
HIV Infections / immunology
Humans
Interleukin-2 Receptor alpha Subunit / blood
Interleukin-2 Receptor alpha Subunit / immunology
Interleukin-7 Receptor alpha Subunit / blood
Interleukin-7 Receptor alpha Subunit / immunology
Male
T-Lymphocytes, Regulatory*

Substances

CD4 Antigens
Interleukin-2 Receptor alpha Subunit
Interleukin-7 Receptor alpha Subunit