Abstract
The present report was undertaken in an effort to characterize the nature of Ca2+ binding protein(s) in the central nervous system of less evolved vertebrates. In particular we investigated whether the brain microsomal fraction of Rana esculenta expresses calsequestrin, calreticulin and/or other related Ca2+ binding protein(s). We found that a 60 KDa protein having an NH2-terminal amino acid sequence similar to mammalian calreticulin is the major microsomal Ca2(+)-binding protein.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Blotting, Western
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Brain / physiology
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Calcium-Binding Proteins / chemistry
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Calcium-Binding Proteins / metabolism*
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Calreticulin
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Calsequestrin / chemistry
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Calsequestrin / metabolism
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Electrophoresis, Polyacrylamide Gel
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Molecular Sequence Data
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Rana esculenta / physiology*
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Species Specificity
Substances
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Calcium-Binding Proteins
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Calreticulin
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Calsequestrin