A new triterpene glycoside mutongsaponin F (1), together with five known saponins and two known lipids, was isolated from the 70% ethanol extract of the stems of Akebia trifoliata (Thunb.) Koidz. var. australis (Diels) Rehd. Their structures were elucidated on the basis of the spectroscopic analysis and physicochemical properties as 3-beta-[(beta-D-glucopyranosyl-(1-->2)-O-[beta-D-glucopyranosyl-(1-->3)-O-]-alpha-L-arabinopyranosyl)oxy]-30-norolean-12-en-28-oic acid alpha-L-rhamnopyranosyl-(1-->4)-O-beta-D-glucopyranosyl-(1-->6)-O-beta-D-glucopyranosyl ester (1), 3-beta-[(beta-D-glucopyranosyl-(1-->2)-O-[beta-D-glucopyranosyl-(1-->3)-O-]-alpha-L-arabinopyranosyl)oxy]-30-norolean-12-en-28-oic acid (2), leonticin E (3), collinsonidin (4), arjunolic acid 28-O-glucopyranoside (5), asiatic acid 28-O-glucopyranoside (6), soya-cerebroside I (7), and 1-O-alpha-L-galactosyl-(1-->6)-O-beta-D-galactosyl-3-O-hexadecanoyl-glycerol (8), respectively.