Molecular cloning of CD18 of bison, deer and elk, and comparison with that of other ruminants and non-ruminants

Abstract

Pneumonia caused by Mannheimia haemolytica is an important disease of cattle, domestic sheep, bighorn sheep and goats. Leukotoxin (Lkt) produced by M. haemolytica is cytolytic to all leukocyte subsets of these species. Lkt utilizes CD18, the beta subunit of beta(2)-integrins, as its functional receptor on leukocytes of these species. Cytotoxicity assays revealed that leukocytes from bison, deer, and elk are also susceptible to Lkt-induced cytolysis. The availability of cDNA encoding CD18 of bison, deer and elk would facilitate the comparison of a greater number of ruminant CD18 cDNA with that of non-ruminants as a means of the elucidation of the molecular basis for the specificity of M. haemolytica Lkt for ruminant leukocytes. Herein, we report the cloning and characterization of bison, deer, and elk CD18. The full-length cDNA of bison and deer consists of 2310bp with an ORF encoding 769 amino acids while elk CD18 consists of 2313bp with an ORF encoding 770 amino acids. This gene is highly conserved among ruminants compared with non-ruminants. Phylogenetic analysis based on amino acid sequences showed that CD18 of bison is most closely related to that of cattle while CD18 of deer and elk are more closely related to each other.