Improved poly-gamma-glutamic acid production by chromosomal integration of the Vitreoscilla hemoglobin gene (vgb) in Bacillus subtilis

Yishan Su; Xia Li; Qingzhi Liu; Zhongwen Hou; Xiqiang Zhu; Xueping Guo; Peixue Ling

doi:10.1016/j.biortech.2010.01.128

Improved poly-gamma-glutamic acid production by chromosomal integration of the Vitreoscilla hemoglobin gene (vgb) in Bacillus subtilis

Bioresour Technol. 2010 Jun;101(12):4733-6. doi: 10.1016/j.biortech.2010.01.128. Epub 2010 Feb 19.

Authors

Yishan Su¹, Xia Li, Qingzhi Liu, Zhongwen Hou, Xiqiang Zhu, Xueping Guo, Peixue Ling

Affiliation

¹ Shandong Academy of Pharmaceutical Science, Jinan 250101, PR China.

PMID: 20171882
DOI: 10.1016/j.biortech.2010.01.128

Abstract

In order to alleviate oxygen limitation and improve the yield of poly-gamma-glutamic acid (gamma-PGA) during fermentation, the Vitreoscilla hemoglobin gene (vgb) was integrated into the chromosome of Bacillus subtilis and expressed during gamma-PGA production. The activity of the expressed Vitreoscilla hemoglobin (VHb) was confirmed by CO-difference spectrum. Expression of VHb enhanced cell growth under high viscosity fermentation conditions 1.26-fold and increased the yield of gamma-PGA 2.07-fold. These results indicate that the expression of VHb could be advantageous in high viscosity fermentation media.

MeSH terms

Bacillus subtilis / growth & development
Bacillus subtilis / metabolism*
Bacterial Proteins / genetics*
Chromosomes, Bacterial / genetics*
Genes, Bacterial / genetics*
Mutagenesis, Insertional / genetics*
Polyglutamic Acid / analogs & derivatives*
Polyglutamic Acid / biosynthesis
Polymerase Chain Reaction
Reproducibility of Results
Spectrum Analysis
Time Factors
Transformation, Genetic
Truncated Hemoglobins / genetics*

Substances

Bacterial Proteins
Truncated Hemoglobins
poly(gamma-glutamic acid)
hemoglobin protein, Vitreoscilla
Polyglutamic Acid