Synthesis and AFM characterization of poly(dG)-poly(dC)-gold nanoparticle conjugates

Dragoslav Zikich; Natalia Borovok; Tatiana Molotsky; Alexander Kotlyar

doi:10.1021/bc900527a

Synthesis and AFM characterization of poly(dG)-poly(dC)-gold nanoparticle conjugates

Bioconjug Chem. 2010 Mar 17;21(3):544-7. doi: 10.1021/bc900527a. Epub 2010 Feb 18.

Authors

Dragoslav Zikich¹, Natalia Borovok, Tatiana Molotsky, Alexander Kotlyar

Affiliation

¹ Department of Biochemistry, George S. Wise Faculty of Life Sciences, Tel Aviv University, The Center for Nanoscience and Nanotechnology, Tel Aviv University, Ramat Aviv, 69978.

PMID: 20166677
DOI: 10.1021/bc900527a

Abstract

In the present work, we have synthesized conjugates between the 5 nm gold nanoparticles (Au-NP) and 5' thiol-functionalized, 700 bp poly(dG)-poly(dC). We have completely separated and purified to homogeneity conjugates bearing different number of poly(dG)-poly(dC) molecules per Au-NP by electrophoresis and HPLC. The conjugates were directly visualized by atomic force microscopy. We have demonstrated that Au NP-bound poly(dG)-poly(dC) can be considerably extended by Klenow exo(-) polymerase in the presence of dCTP and dGTP.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, High Pressure Liquid
Electrophoresis, Agar Gel
Gold / chemistry*
Metal Nanoparticles / chemistry*
Microscopy, Atomic Force
Particle Size
Polydeoxyribonucleotides / chemical synthesis*
Polydeoxyribonucleotides / chemistry*
Surface Properties

Substances

Polydeoxyribonucleotides
poly(dG).poly(dC)
Gold