Matrix-assisted laser desorption ionization-time of flight mass spectrometry for direct bacterial identification from positive blood culture pellets

J Clin Microbiol. 2010 Apr;48(4):1481-3. doi: 10.1128/JCM.01780-09. Epub 2010 Feb 17.

Abstract

An ammonium chloride erythrocyte-lysing procedure was used to prepare a bacterial pellet from positive blood cultures for direct matrix-assisted laser desorption-ionization time of flight (MALDI-TOF) mass spectrometry analysis. Identification was obtained for 78.7% of the pellets tested. Moreover, 99% of the MALDI-TOF identifications were congruent at the species level when considering valid scores. This fast and accurate method is promising.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteremia / diagnosis*
  • Bacteremia / microbiology*
  • Bacteria / chemistry
  • Bacteria / classification*
  • Bacteria / isolation & purification*
  • Bacteriological Techniques / methods*
  • Blood / microbiology*
  • Humans
  • Sensitivity and Specificity
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
  • Time Factors