Bioconversion of the antihistaminc drug loratadine by tobacco cell suspension cultures expressing human cytochrome P450 3A4

Heribert Warzecha; Daniela Ferme; Markus Peer; Andreas Frank; Matthias Unger

doi:10.1016/j.jbiosc.2009.09.001

Bioconversion of the antihistaminc drug loratadine by tobacco cell suspension cultures expressing human cytochrome P450 3A4

J Biosci Bioeng. 2010 Mar;109(3):288-90. doi: 10.1016/j.jbiosc.2009.09.001. Epub 2009 Sep 29.

Authors

Heribert Warzecha¹, Daniela Ferme, Markus Peer, Andreas Frank, Matthias Unger

Affiliation

¹ Technische Universitaet Darmstadt, Department for Biology, Schnittspahnstr.3-5, 65287 Darmstadt, Germany. warzecha@bio.tu-darmstadt.de

PMID: 20159579
DOI: 10.1016/j.jbiosc.2009.09.001

Abstract

In this study we have expanded the metabolic potential of plant cell suspension cultures by introducing active human cytochrome P450 monooxygenase 3A4 into tobacco cells. Exogenously supplied loratadine was metabolized in a 3A4-specific manner, showing the capacity of this system for the generation of metabolites.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cytochrome P-450 CYP3A / genetics*
Cytochrome P-450 CYP3A / metabolism*
Histamine Antagonists / metabolism*
Humans
Loratadine / metabolism*
Nicotiana / physiology*
Plants, Genetically Modified / metabolism*
Protein Engineering / methods*
Recombinant Proteins / metabolism

Substances

Histamine Antagonists
Recombinant Proteins
Loratadine
Cytochrome P-450 CYP3A
CYP3A4 protein, human