Temporal pixel multiplexing for simultaneous high-speed, high-resolution imaging

Nat Methods. 2010 Mar;7(3):209-11. doi: 10.1038/nmeth.1429. Epub 2010 Feb 14.

Abstract

We introduce an imaging modality that, by offsetting pixel-exposure times during capture of a single image frame, embeds temporal information in each frame. This allows simultaneous acquisition of full-resolution images at native detector frame rates and high-speed image sequences at reduced resolution, without increasing bandwidth requirements. We demonstrate this method using macroscopic and microscopic examples, including imaging calcium transients in heart cells at 250 Hz using a 10-Hz megapixel camera.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium / metabolism
  • Diagnostic Imaging / methods*
  • Image Interpretation, Computer-Assisted
  • Microscopy, Fluorescence
  • Rats
  • Signal Processing, Computer-Assisted

Substances

  • Calcium