The secretome of a recombinant BCG substrain reveals differences in hypothetical proteins

Mauricio Rodríguez-Alvarez; Iliana D Palomec-Nava; Guillermo Mendoza-Hernández; Yolanda López-Vidal

doi:10.1016/j.vaccine.2010.01.064

The secretome of a recombinant BCG substrain reveals differences in hypothetical proteins

Vaccine. 2010 May 21;28(23):3997-4001. doi: 10.1016/j.vaccine.2010.01.064. Epub 2010 Feb 12.

Authors

Mauricio Rodríguez-Alvarez¹, Iliana D Palomec-Nava, Guillermo Mendoza-Hernández, Yolanda López-Vidal

Affiliation

¹ Programa de Inmunología Molecular Microbiana, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), Edificio de Investigación, 4 degrees piso, Av. Universidad #3000, Coyoacán, 04510 México, D.F., Mexico.

PMID: 20153797
DOI: 10.1016/j.vaccine.2010.01.064

Abstract

Tuberculosis remains a major human health problem worldwide, and strategies for its prevention include the generation and characterization of new recombinant vaccines containing immunodominant antigens from Mycobacterium tuberculosis. By comparing the secretomes of wild-type Mycobacterium bovis and a PstS1-recombinant M. bovis BCG vaccine substrain (rBCG38), we identified six conserved hypothetical proteins (BCG2696, BCG1674, BCG0372, BCG0427, BCG2436c, and BCG3053) that are differentially expressed. Our findings will aid in the identification of highly immunogenic proteins present in rBCG.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

BCG Vaccine / immunology*
Bacterial Proteins / immunology*
Immunodominant Epitopes / immunology*
Isoelectric Focusing
Mycobacterium bovis / metabolism
Tuberculosis / immunology
Tuberculosis / prevention & control
Vaccines, Synthetic / immunology

Substances

BCG Vaccine
Bacterial Proteins
Immunodominant Epitopes
Vaccines, Synthetic