Abstract
Proteins with Pumilio RNA binding domains (Puf proteins) are ubiquitous in eukaryotes. Some Puf proteins bind to the 3'-untranslated regions of mRNAs, acting to repress translation and promote degradation; others are involved in ribosomal RNA maturation. The genome of Trypanosoma brucei encodes eleven Puf proteins whose function cannot be predicted by sequence analysis. We show here that epitope-tagged TbPUF7 is located in the nucleolus, and associated with a nuclear cyclophilin-like protein, TbNCP1. RNAi targeting PUF7 reduced trypanosome growth and inhibited two steps in ribosomal RNA processing.
Copyright 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Nucleus / metabolism
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Cyclophilins / metabolism*
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Growth and Development / drug effects
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Growth and Development / genetics
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Phylogeny
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Protein Binding / physiology
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Protein Structure, Tertiary
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RNA Processing, Post-Transcriptional* / drug effects
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RNA Processing, Post-Transcriptional* / genetics
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RNA, Ribosomal / metabolism*
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RNA, Small Interfering / pharmacology
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RNA-Binding Proteins / antagonists & inhibitors
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RNA-Binding Proteins / genetics
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RNA-Binding Proteins / metabolism*
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RNA-Binding Proteins / physiology*
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / growth & development
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Saccharomyces cerevisiae / metabolism
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Sequence Alignment
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Sequence Homology
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Trypanosoma brucei brucei / genetics
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Trypanosoma brucei brucei / growth & development
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Trypanosoma brucei brucei / metabolism
Substances
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RNA, Ribosomal
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RNA, Small Interfering
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RNA-Binding Proteins
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Cyclophilins