Effect of 3'-end capping of aptamer with various 2',4'-bridged nucleotides: Enzymatic post-modification toward a practical use of polyclonal aptamers

Yuuya Kasahara; Shunsuke Kitadume; Kunihiko Morihiro; Masayasu Kuwahara; Hiroaki Ozaki; Hiroaki Sawai; Takeshi Imanishi; Satoshi Obika

doi:10.1016/j.bmcl.2010.01.028

Effect of 3'-end capping of aptamer with various 2',4'-bridged nucleotides: Enzymatic post-modification toward a practical use of polyclonal aptamers

Bioorg Med Chem Lett. 2010 Mar 1;20(5):1626-9. doi: 10.1016/j.bmcl.2010.01.028. Epub 2010 Jan 22.

Authors

Yuuya Kasahara¹, Shunsuke Kitadume, Kunihiko Morihiro, Masayasu Kuwahara, Hiroaki Ozaki, Hiroaki Sawai, Takeshi Imanishi, Satoshi Obika

Affiliation

¹ Department of Chemistry and Chemical Biology, Graduate School of Engineering, Gunma University, Gunma 376-8515, Japan.

PMID: 20153191
DOI: 10.1016/j.bmcl.2010.01.028

Abstract

The capping of the 3'-ends of thrombin binding aptamers (TBAs) with bridged nucleotides increased the nuclease resistances and the stabilities in human serum. The binding abilities of the aptamers were not affected by the capping. The capping could be simply executed via a one step enzymatic process using 2',4'-bridged nucleoside 5'-triphosphate and terminal deoxynucleotidyl transferase.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aptamers, Nucleotide / chemistry*
Aptamers, Nucleotide / metabolism
DNA Nucleotidylexotransferase / metabolism
Humans
Nucleotides / chemistry*
Phosphodiesterase I / metabolism
Protein Binding
Protein Processing, Post-Translational
Protein Stability
Time Factors

Substances

Aptamers, Nucleotide
Nucleotides
thrombin aptamer
DNA Nucleotidylexotransferase
Phosphodiesterase I