Use of the Sleeping Beauty transposon system for stable gene expression in mouse embryonic stem cells

Cold Spring Harb Protoc. 2009 Aug;2009(8):pdb.prot5270. doi: 10.1101/pdb.prot5270.
No abstract available

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Binding Sites / genetics
  • Cell Line
  • DNA Transposable Elements / genetics*
  • Embryonic Stem Cells / cytology
  • Embryonic Stem Cells / metabolism*
  • Gene Expression*
  • Mice
  • Models, Genetic
  • Mutagenesis, Insertional
  • Transfection / methods

Substances

  • DNA Transposable Elements