Abstract
Biotech crops expressing Bacillus thuringiensis Cry toxins present a valuable approach for insect control. Cry8Ka5, which is highly toxic to the cotton boll weevil (Anthonomus grandis), was used as a model to study toxin-ligand interactions. Three Cry-binding proteins were detected after toxin overlay assays. Following de novo sequencing, a heat-shock cognate protein and a V-ATPase were identified, whilst a approximately 120 kDa protein remained unknown. Additional Cry8Ka5-binding proteins were visualized by two-dimensional gel electrophoresis ligand blots.
(c) 2010 Elsevier Inc. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bacillus thuringiensis / metabolism*
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Bacillus thuringiensis Toxins
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Bacterial Proteins / chemistry
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Bacterial Proteins / metabolism*
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Digestive System / metabolism*
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Endotoxins / chemistry
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Endotoxins / metabolism*
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HSC70 Heat-Shock Proteins / analysis
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HSC70 Heat-Shock Proteins / metabolism
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Hemolysin Proteins / chemistry
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Hemolysin Proteins / metabolism*
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Insecticides / chemistry
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Insecticides / metabolism*
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Larva / metabolism
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Pest Control, Biological / methods*
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Protein Binding
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Vacuolar Proton-Translocating ATPases / analysis
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Vacuolar Proton-Translocating ATPases / metabolism
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Weevils / metabolism*
Substances
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Bacillus thuringiensis Toxins
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Bacterial Proteins
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Endotoxins
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HSC70 Heat-Shock Proteins
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Hemolysin Proteins
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Insecticides
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insecticidal crystal protein, Bacillus Thuringiensis
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Vacuolar Proton-Translocating ATPases