Abstract
This paper describes cloning, expression, characterization and crystallization of feruloyl esterase from Aspergillus awamori. The gene coding for feruloyl esterase from A. awamori was amplified, cloned, and expressed in Escherichia coli. The recombinant enzyme was purified, characterized and used for crystallization. Its X-ray crystallographic data were collected and explained using molecular replacement theory. Also, the tertiary structure of the recombinant enzyme-ferulic acid complex was also determined.
2010 Elsevier B.V. All rights reserved.
MeSH terms
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Aspergillus / enzymology
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Aspergillus / genetics*
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Carboxylic Ester Hydrolases / biosynthesis
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Carboxylic Ester Hydrolases / chemistry*
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Carboxylic Ester Hydrolases / genetics*
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Carboxylic Ester Hydrolases / isolation & purification
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Cloning, Molecular
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Crystallography, X-Ray
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DNA, Ribosomal / metabolism
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Escherichia coli / genetics*
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Gene Expression
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Models, Molecular
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Molecular Sequence Data
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Protein Conformation
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / chemistry*
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Recombinant Proteins / genetics*
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Recombinant Proteins / isolation & purification
Substances
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DNA, Ribosomal
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Recombinant Proteins
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Carboxylic Ester Hydrolases
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feruloyl esterase