Abstract
The effects of diclazuril on mRNA expression levels of invasion-related microneme genes were examined in second-generation merozoites of Eimeria tenella (E. tenella) by quantitative real-time (QRT) PCR. Diclazruil treatment of infected chickens significantly decreased the number of second-generation merozoites by 65.13%, and resulted in downregulation of EtMIC genes: EtMIC1 by 65.63%, EtMIC2 by 64.12%, EtMIC3 by 56.82%, EtMIC4 by 73.48%, and EtMIC5 by 78.17%. SEM images of caecum tissue from uninfected chickens showed regular intestinal villus structure. In infected chickens, a distinct loss of the superficial epithelium, with a flattened mucosa and large-area necrosis and anabrosis, was evident. In diclazruil-treated chickens, a decrease in merozoite number and a visibly improved appearance of the caeca were noted. These improvements appeared to be mediated in part by downregulation of the expression of invasion-related EtMIC genes in response to diclazuril.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Cecum / parasitology
-
Cecum / pathology
-
Cecum / ultrastructure
-
Chickens / parasitology*
-
Coccidiosis / drug therapy
-
Coccidiosis / parasitology
-
Coccidiosis / pathology
-
Coccidiosis / veterinary*
-
Coccidiostats / pharmacology
-
Coccidiostats / therapeutic use*
-
DNA, Complementary / metabolism
-
Eimeria tenella / drug effects*
-
Eimeria tenella / genetics
-
Eimeria tenella / pathogenicity
-
Gene Expression / drug effects
-
Glycoproteins / genetics
-
Glycoproteins / metabolism
-
Male
-
Merozoites / drug effects
-
Merozoites / metabolism
-
Microscopy, Electron, Scanning
-
Nitriles / pharmacology
-
Nitriles / therapeutic use*
-
Polymerase Chain Reaction
-
Poultry Diseases / drug therapy*
-
Poultry Diseases / parasitology
-
Poultry Diseases / pathology
-
Protozoan Proteins / genetics
-
Protozoan Proteins / metabolism
-
RNA, Protozoan / genetics
-
RNA, Protozoan / isolation & purification
-
Random Allocation
-
Triazines / pharmacology
-
Triazines / therapeutic use*
Substances
-
Coccidiostats
-
DNA, Complementary
-
Glycoproteins
-
Nitriles
-
Protozoan Proteins
-
RNA, Protozoan
-
Triazines
-
microneme protein 5, Eimeria tenella
-
diclazuril