Analysis of the degradome with the CLIP-CHIP microarray

Reinhild Kappelhoff; Ulrich Auf dem Keller; Christopher M Overall

doi:10.1007/978-1-60327-299-5_10

Analysis of the degradome with the CLIP-CHIP microarray

Methods Mol Biol. 2010:622:175-93. doi: 10.1007/978-1-60327-299-5_10.

Authors

Reinhild Kappelhoff¹, Ulrich Auf dem Keller, Christopher M Overall

Affiliation

¹ Department of Oral Biological and Medical Sciences, Centre for Blood Research, University of British Columbia, Vancouver, BC, Canada.

PMID: 20135282
DOI: 10.1007/978-1-60327-299-5_10

Abstract

The degradome microarray - CLIP-CHIP - is a dedicated and focused array that allows the analysis of all proteases, non-proteolytic homologs, and protease inhibitor gene transcripts in the human and murine genomes at the mRNA transcript level.Based on unique 70-mer oligonucleotides, designed to match parts of the sequence of known or predicted protease and inhibitor mRNAs in both species and printed on a glass-matrix surface, the CLIP-CHIP microarray can be used to analyze differentially expressed protease and inhibitor gene products and give expression profiles for any analyzed sample.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Fluorescent Dyes / metabolism
Humans
Image Processing, Computer-Assisted
Mice
Nucleic Acid Hybridization
Oligonucleotide Array Sequence Analysis / methods*
Protein Processing, Post-Translational*
Proteome / analysis*
RNA / isolation & purification
Statistics as Topic

Substances

Fluorescent Dyes
Proteome
RNA