Abstract
High-resolution melt (HRM) analysis can identify sequence polymorphisms by comparing the melting curves of amplicons generated by real-time PCR amplification. We describe the application of this technique to identify Mycobacterium avium subspecies paratuberculosis types I, II, and III. The HRM approach was based on type-specific nucleotide sequences in MAP1506, a member of the PPE (proline-proline-glutamic acid) gene family.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics*
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Mycobacterium avium subsp. paratuberculosis / classification*
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Mycobacterium avium subsp. paratuberculosis / genetics
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Mycobacterium avium subsp. paratuberculosis / isolation & purification*
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Paratuberculosis / diagnosis*
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Paratuberculosis / microbiology
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Polymerase Chain Reaction / methods*
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Transition Temperature*