Abstract
Polystyrene-supported 2-isobutoxy-1-isobutoxycarbonyl-1,2-dihydroquinoline (PS-IIDQ), a polymer-supported covalent coupling reagent, was successfully employed for the first time in the bioconjugation of an example hapten (phytanic acid derivative) to a carrier protein (bovine serum albumin (BSA)) within the context of immunogen preparation for antibody development. The ability of the prepared example phytanic acid derivative-BSA conjugate to bind an anti-phytanic acid antibody was confirmed using an enzyme-linked immunosorbent assay (ELISA).
Copyright 2010 Elsevier Ltd. All rights reserved.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Antibodies / metabolism*
-
Cattle
-
Enzyme-Linked Immunosorbent Assay
-
Haptens / chemistry
-
Haptens / immunology*
-
Phytanic Acid / analogs & derivatives*
-
Phytanic Acid / chemical synthesis
-
Phytanic Acid / chemistry
-
Phytanic Acid / immunology
-
Phytanic Acid / pharmacology
-
Polystyrenes / chemistry*
-
Quinolines / chemistry*
-
Serum Albumin, Bovine / chemical synthesis
-
Serum Albumin, Bovine / chemistry*
-
Serum Albumin, Bovine / immunology*
-
Serum Albumin, Bovine / pharmacology
-
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Substances
-
Antibodies
-
Haptens
-
Polystyrenes
-
Quinolines
-
Phytanic Acid
-
Serum Albumin, Bovine
-
IIDQ