Abstract
Stem cell-based therapy using bone marrow stromal cells (MSCs) has been expected to be a promising therapy for neuronal regeneration. To repair the injured spinal cord, neuronal differentiation of MSCs before transplantation has a more satisfactory effect. Recently, neuronal differentiation of neural progenitor/stem cells by an intracellular delivery of a pVHL-derived synthetic peptide (VHL peptide) has been shown. Here, we show that VHL peptide-delivered MSCs differentiated into neuron-like cells, and that engrafted VHL peptide-delivered MSCs more recovered the behaviors of the rats than that of nondelivered MSCs. Our result suggests that the use of VHL peptide-delivered MSCs would be a promising therapeutic strategy for repairing the injured spinal cord.
MeSH terms
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Amino Acid Sequence
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Animals
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Blotting, Western
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Bone Marrow Cells / cytology
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Bone Marrow Cells / metabolism
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Cell Culture Techniques
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Cell Differentiation
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Disease Models, Animal
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Immunohistochemistry
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Male
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Microtubule-Associated Proteins / genetics
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Microtubule-Associated Proteins / metabolism
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Nerve Regeneration*
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Neurofilament Proteins / genetics
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Neurofilament Proteins / metabolism
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Peptide Biosynthesis / genetics
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Peptide Fragments / genetics
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Peptide Fragments / metabolism
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Peptide Fragments / pharmacology*
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Rats
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Rats, Wistar
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Recombinant Fusion Proteins / chemical synthesis
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / pharmacology
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Recovery of Function*
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Reverse Transcriptase Polymerase Chain Reaction
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Spinal Cord Injuries / metabolism
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Spinal Cord Injuries / physiopathology
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Spinal Cord Injuries / surgery*
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Stem Cell Transplantation / methods*
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Stem Cells / cytology
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Stem Cells / drug effects
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Stem Cells / metabolism
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Transduction, Genetic / methods
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Von Hippel-Lindau Tumor Suppressor Protein / genetics
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Von Hippel-Lindau Tumor Suppressor Protein / metabolism
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Von Hippel-Lindau Tumor Suppressor Protein / pharmacology*
Substances
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MAP2 protein, rat
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Microtubule-Associated Proteins
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Neurofilament Proteins
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Peptide Fragments
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Recombinant Fusion Proteins
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neurofilament protein H
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Von Hippel-Lindau Tumor Suppressor Protein