Cell-laden microwells for the study of multicellularity in lymphocyte fate decisions

Junsang Doh; Miju Kim; Matthew F Krummel

doi:10.1016/j.biomaterials.2010.01.018

Cell-laden microwells for the study of multicellularity in lymphocyte fate decisions

Biomaterials. 2010 Apr;31(12):3422-8. doi: 10.1016/j.biomaterials.2010.01.018. Epub 2010 Feb 1.

Authors

Junsang Doh¹, Miju Kim, Matthew F Krummel

Affiliation

¹ School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang, Gyeongbuk, Republic of Korea. jsdoh@postech.ac.kr

PMID: 20117834
DOI: 10.1016/j.biomaterials.2010.01.018

Abstract

Cell-cell cooperativity in populations of motile and transiently interacting immune cells has been difficult to assess in the absence of tools to control proximity and communication. Here, we describe the generation of cell-laden microwells that can precisely control contact-mediated interactions and multicellular 'quorum' decisions in lymphocytes. Different types of fate decisions for activating T cells can be shown to variously obey 'binary' or 'density' outcomes, correlated with cell-cell contact, using this new platform.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CD4-Positive T-Lymphocytes / cytology*
Cell Lineage*
Humans
Lymphocyte Activation