Characterization of a human TAR RNA-binding protein that activates the HIV-1 LTR

Science. 1991 Mar 29;251(5001):1597-600. doi: 10.1126/science.2011739.

Abstract

Human immunodeficiency virus type 1 (HIV-1) gene expression is activated by Tat, a virally encoded protein. Tat trans-activation requires viral (trans-activation--responsive; TAR) RNA sequences located in the R region of the long terminal repeat (LTR). Existing evidence suggests that Tat probably cooperates with cellular factors that bind to TAR RNA in the overall trans-activation process. A HeLa complementary DNA was isolated and characterized that encodes a TAR RNA-binding protein (TRBP). TRBP activated the HIV-1 LTR and was synergistic with Tat function.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Binding Sites
  • Carrier Proteins / genetics*
  • Endoribonucleases / genetics
  • Escherichia coli / enzymology
  • Escherichia coli Proteins*
  • Gene Products, tat / metabolism
  • HIV Long Terminal Repeat*
  • HIV-1 / genetics*
  • Humans
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • Plasmids
  • RNA, Viral / genetics
  • RNA-Binding Proteins*
  • Ribonuclease III
  • Sequence Homology, Nucleic Acid
  • tat Gene Products, Human Immunodeficiency Virus

Substances

  • Carrier Proteins
  • Escherichia coli Proteins
  • Gene Products, tat
  • RNA, Viral
  • RNA-Binding Proteins
  • tat Gene Products, Human Immunodeficiency Virus
  • trans-activation responsive RNA-binding protein
  • Endoribonucleases
  • Ribonuclease III
  • ribonuclease III, E coli

Associated data

  • GENBANK/M55283
  • GENBANK/M60801
  • GENBANK/M60861
  • GENBANK/S71725
  • GENBANK/X53015
  • GENBANK/X53016
  • GENBANK/X53017
  • GENBANK/X53018
  • GENBANK/X53019
  • GENBANK/X53020
  • GENBANK/X53021