Abstract
Human immunodeficiency virus type 1 (HIV-1) gene expression is activated by Tat, a virally encoded protein. Tat trans-activation requires viral (trans-activation--responsive; TAR) RNA sequences located in the R region of the long terminal repeat (LTR). Existing evidence suggests that Tat probably cooperates with cellular factors that bind to TAR RNA in the overall trans-activation process. A HeLa complementary DNA was isolated and characterized that encodes a TAR RNA-binding protein (TRBP). TRBP activated the HIV-1 LTR and was synergistic with Tat function.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Binding Sites
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Carrier Proteins / genetics*
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Endoribonucleases / genetics
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Escherichia coli / enzymology
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Escherichia coli Proteins*
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Gene Products, tat / metabolism
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HIV Long Terminal Repeat*
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HIV-1 / genetics*
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Humans
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Molecular Sequence Data
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Nucleic Acid Conformation
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Plasmids
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RNA, Viral / genetics
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RNA-Binding Proteins*
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Ribonuclease III
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Sequence Homology, Nucleic Acid
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tat Gene Products, Human Immunodeficiency Virus
Substances
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Carrier Proteins
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Escherichia coli Proteins
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Gene Products, tat
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RNA, Viral
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RNA-Binding Proteins
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tat Gene Products, Human Immunodeficiency Virus
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trans-activation responsive RNA-binding protein
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Endoribonucleases
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Ribonuclease III
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ribonuclease III, E coli
Associated data
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GENBANK/M55283
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GENBANK/M60801
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GENBANK/M60861
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GENBANK/S71725
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GENBANK/X53015
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GENBANK/X53016
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GENBANK/X53017
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GENBANK/X53018
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GENBANK/X53019
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GENBANK/X53020
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GENBANK/X53021