Herd-level factors for Brucella seropositivity in cattle reared in smallholder dairy farms of Zimbabwe

Abstract

A cross-sectional study was conducted to investigate factors for Brucella seropositivity in smallholder dairy cattle herds from Gokwe, Marirangwe, Mushagashe, Nharira, Rusitu and Wedza areas located in different agro-ecological regions of Zimbabwe between September 2004 and November 2005. Sera were collected from cattle aged > or = 2 years from 203 herds. Data on herd-level and management variables were collected using a structured questionnaire. Sera were screened for anti-Brucella antibodies using the Rose Bengal test (RBT) and confirmed by competitive ELISA (c-ELISA). A herd was classified as Brucella seropositive if at least one animal tested seropositive on both tests. The herd-level factors for Brucella seropositivity were tested using multivariable logistic model with herd infection status as dependent variable while the levels of exposure in individual animals within-herds were analysed by negative binomial regression using the number of positive animals as the outcome. Of the 203 herds tested, 52 (25; 95% CI: 18.1, 31.9%) tested seropositive for brucellosis. Using the logistic regression model we identified area, with both Rusitu (OR=0.26; 95% CI: 0.07, 1.03) and Wedza (OR=0.07; 95% CI: 0.01, 0.49) having lower Brucella seropositivity compared to Gokwe. Keeping mixed cattle breeds (OR=8.33; 95% CI: 2.70, 25.72) compared to single breed herds, was associated with increased herd seropositivity. The farmer's knowledge of brucellosis was associated with lower odds (OR=0.17; 95% CI: 0.05, 0.55) of farms testing seropositive. The odds of Brucella seropositivity were progressively higher with increasing stocking density and herd size. Using the negative binomial regression model we identified area, keeping mixed breed herds, stocking density and herd size as independently associated with increased counts of seropositive cattle in a herd.