Abstract
A Candida glabrata calcineurin mutant exhibited increased susceptibility to both azole antifungal and cell wall-damaging agents and was also attenuated in virulence. Although a mutant lacking the downstream transcription factor Crz1 displayed a cell wall-associated phenotype intermediate to that of the calcineurin mutant and was modestly attenuated in virulence, it did not show increased azole susceptibility. These results suggest that calcineurin regulates both Crz1-dependent and -independent pathways depending on the type of stress.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antifungal Agents / pharmacology*
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Base Sequence
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Calcineurin / genetics
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Calcineurin / metabolism*
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Candida glabrata / drug effects*
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Candida glabrata / genetics
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Candida glabrata / pathogenicity*
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Candida glabrata / physiology
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Candidiasis / drug therapy
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Candidiasis / microbiology
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DNA Primers / genetics
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DNA, Fungal / genetics
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Drug Resistance, Fungal / genetics
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Female
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Fungal Proteins / genetics
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Fungal Proteins / metabolism*
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Genes, Fungal
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Humans
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In Vitro Techniques
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Mice
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Mice, Inbred BALB C
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Microbial Sensitivity Tests
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Mutation
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Opportunistic Infections / drug therapy
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Opportunistic Infections / microbiology
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Virulence / genetics
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Virulence / physiology
Substances
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Antifungal Agents
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DNA Primers
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DNA, Fungal
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Fungal Proteins
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Transcription Factors
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Calcineurin