Immunological studies of the toxic site in ammodytoxin A

FEBS Lett. 1991 Mar 11;280(1):175-8. doi: 10.1016/0014-5793(91)80231-q.

Abstract

Two monoclonal antibodies against the native ammodytoxin A and four site-directed polyclonal antibodies against synthetic peptides derived from the primary structure of the toxin were prepared in order to estimate the localization of its toxic site. Some of the antibodies neutralized the lethal toxicity of the toxin, thus indicating an approximate position of the toxic or receptor binding site on the molecule that is different from those predicted by comparison with a number of known sequences.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal / immunology
  • Antigens / immunology
  • Binding Sites
  • Cytotoxicity Tests, Immunologic
  • Female
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Phospholipases A / chemistry*
  • Phospholipases A / immunology
  • Protein Conformation
  • Stereoisomerism
  • Viper Venoms / chemistry*
  • Viper Venoms / immunology

Substances

  • Antibodies, Monoclonal
  • Antigens
  • Viper Venoms
  • ammodytoxin A
  • Phospholipases A