Abstract
Edaravone has been reported to have a radioprotective effect at high concentrations. We now report that a lower dose of edaravone enhanced X-ray-induced apoptosis of some cell lines harboring p53 wild-type status, such as MOLT-4, Nalm-6, and HepG2. The knock-down of p53 using siRNA in MOLT-4 cells abolished the radiosensitizing effect of edaravone. Enhanced phosphorylations of p53 at Ser 15 and Ser 20 and up-regulation of PUMA, a p53 target protein, were observed after X-irradiation in the presence of edaravone. We conclude that the low dose of edaravone sensitized cells to X-irradiation by promoting the p53-dependent apoptotic signaling pathway.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antipyrine / analogs & derivatives*
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Antipyrine / pharmacology
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Apoptosis / drug effects*
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Apoptosis / radiation effects
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Cell Line, Tumor
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Dose-Response Relationship, Drug
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Edaravone
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Free Radical Scavengers / pharmacology*
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Gene Knockdown Techniques
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Hep G2 Cells
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Humans
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Leukemia, T-Cell / drug therapy
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Leukemia, T-Cell / metabolism
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Leukemia, T-Cell / pathology
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Leukemia, T-Cell / radiotherapy
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Radiation-Sensitizing Agents / pharmacology
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Reactive Oxygen Species / metabolism
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Transfection
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Tumor Cells, Cultured
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Tumor Suppressor Protein p53 / deficiency
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Tumor Suppressor Protein p53 / genetics
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Tumor Suppressor Protein p53 / metabolism
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X-Rays
Substances
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Free Radical Scavengers
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Radiation-Sensitizing Agents
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Reactive Oxygen Species
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TP53 protein, human
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Tumor Suppressor Protein p53
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Edaravone
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Antipyrine