Abstract
2,4,6-Trinitrotoluene ((15)N or (13)C labeled) was added to Norfolk Harbor sediments to test whether anaerobic bacteria use TNT for growth. Stable-isotope probing (SIP)-terminal restriction fragment length polymorphism (TRFLP) detected peaks in the [(15)N]TNT cultures (60, 163, and 168 bp). The 60-bp peak was also present in the [(13)C]TNT cultures and was related to Lysobacter taiwanensis.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Bacteria, Anaerobic / isolation & purification*
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Bacteria, Anaerobic / metabolism*
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Carbon Isotopes / metabolism*
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DNA Fingerprinting
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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DNA, Ribosomal / chemistry
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DNA, Ribosomal / genetics
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Geologic Sediments*
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Lysobacter / genetics
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Molecular Sequence Data
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Nitrogen Isotopes / metabolism*
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Polymorphism, Restriction Fragment Length
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RNA, Ribosomal, 16S / genetics
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Sequence Analysis, DNA
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Trinitrotoluene / metabolism*
Substances
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Carbon Isotopes
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DNA, Bacterial
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DNA, Ribosomal
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Nitrogen Isotopes
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RNA, Ribosomal, 16S
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Trinitrotoluene