Isolation and identification of arabinose mycolates of Cell Wall Skeleton (CWS) derived from Mycobacterium bovis BCG Tokyo 172 (SMP-105)

Yuko Uenishi; Naoto Kusunose; Ikuya Yano; Makoto Sunagawa

doi:10.1016/j.mimet.2010.01.003

Isolation and identification of arabinose mycolates of Cell Wall Skeleton (CWS) derived from Mycobacterium bovis BCG Tokyo 172 (SMP-105)

J Microbiol Methods. 2010 Mar;80(3):302-5. doi: 10.1016/j.mimet.2010.01.003. Epub 2010 Jan 15.

Authors

Yuko Uenishi¹, Naoto Kusunose, Ikuya Yano, Makoto Sunagawa

Affiliation

¹ Technology Research & Development Division, Dainippon Sumitomo Pharma Co., Ltd., Konohana-ku, Osaka 554-0022, Japan. yuko-uenishi@ds-pharma.co.jp

PMID: 20079769
DOI: 10.1016/j.mimet.2010.01.003

Abstract

A unique hydrolysis method using a two-layer solution, consisting of diluted hydrochloric acid and toluene was developed to isolate whole arabinose mycolates from the cell wall skeleton of Mycobacterium bovis BCG Tokyo 172 (SMP-105) in order to reveal its pivotal role in enhancing immune responses against tumors.

MeSH terms

Adjuvants, Immunologic / chemistry
Arabinose / analogs & derivatives*
Arabinose / chemistry
Cell Wall Skeleton / chemistry*
Hydrochloric Acid / chemistry
Hydrolysis
Molecular Structure
Mycobacterium bovis / chemistry*
Mycolic Acids* / chemistry
Mycolic Acids* / isolation & purification
Tokyo
Toluene / chemistry

Substances

Adjuvants, Immunologic
Cell Wall Skeleton
Mycolic Acids
Toluene
Arabinose
Hydrochloric Acid