Neosporosis is considered one of the main cause of abortion in dairy cattle in the world. The prevalence of Neospora caninum in wild species has been studied since the coyote (Canis latrans), a North American wild canid specie was discovered as definitive host of this parasite. The aim of the present study was to determine the serum prevalence of N. caninum and T. gondii in wild native canids species from Brazil. Serum samples of 25 crab-eating dogs (Cerdocyon thous), five pampas fox (Lycalopex gymnocercus), six bush dogs (Speothos venaticus) e 14 maned wolves (Chrysocyon brachyurus) were tested. The animals were from zoos and sanctuaries from the states of Parana, Santa Catarina, Rio de Janeiro and the Federal District. The total prevalence obtained for N. caninum was 36% (18/50) and for T. gondii was 40% (20/50). The present study demonstrated for the first time the presence of antibodies to N. caninum in bush dogs and the prevalence found was 33,3% (2/6). This study showed the presence of these protozoans in captive wild canids species and to alert about possible contamination sources.