The purpose of this study was to assess the relationship between heart rate deflection point (HRDP) and ventilatory threshold (VT) to the physiological and performance variables in a relatively large group of trained men rowers. We proposed the hypothesis that physiological and performance variables corresponding to HRDP are not significantly different from corresponding variables at VT, which would justify the use of HRDP as a simple, affordable, and noninvasive method of anaerobic threshold assessment in trained rowers. Eighty-nine trained men rowers (mean ± SD: age 21.2 ± 4.1 years; stature 1.89 ± 0.06 m; body mass 89.2 ± 8.4 kg; VO₂max [maximum oxygen uptake] 5.39 ± 0.62 L/min⁻¹) completed an incremental rowing ergometer exercise test to exhaustion. Three independent, experienced observers determined both HRDP and VT. HRDP was determined by visual and computer-aided regression analyses and was evident in all rowers. The main findings include (a) there is a strong relationship among all observed physiological and performance variables corresponding to HR(HRDP) and HR(VT) (r = 0.79-0.96; p < 0.001) and (b) power output, oxygen uptake, ventilation, tidal volume and breathing rate corresponding to HR(HRDP) and HR(VT) were not significantly different (p ≥ 0.011), whereas HR(HRDP) was slightly but significantly higher than HR(VT) (174.5 vs. 172.8 beats·min⁻¹; p = 0.003). The standard error of the estimate in predicting the HR(VT) based on HR(HRDP) was 5.1 beats·min⁻¹. The subsequent data suggest that, in general, trained rowers may be able to periodically assess their aerobic endurance and evaluate the effects of training programs using the HRDP method.