Abstract
Chemokines and their corresponding receptors participate in transmigration of leukemic cells. ERM (ezrin/radixin/moesin) protein family act as linkers between the plasma membrane and the cytoskeleton and are key component in tumor metastasis. In this study we used the CCR9-expressing acute T lymphocytic cell line MOLT4 as a model and assesssed their morphological and functional changes in response to CCL25. The pseudopodium formed and ERM translocated from the cytoplasm to the cell membrane following treatment with CCL25. Ezrin silencing by miRNA showed that ezrin plays important roles in the polarization and invasive behaviour of MOLT4 cells.
Copyright 2009 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cadherins / metabolism
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Cell Line, Tumor / drug effects
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Cell Line, Tumor / ultrastructure
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Cell Polarity / drug effects
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Cell Polarity / genetics
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Chemokines, CC / pharmacology*
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Chemotaxis, Leukocyte / drug effects
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Chemotaxis, Leukocyte / genetics
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Cytoskeletal Proteins / antagonists & inhibitors
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Cytoskeletal Proteins / genetics
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Cytoskeletal Proteins / metabolism
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Cytoskeletal Proteins / physiology*
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Humans
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Membrane Proteins / genetics
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Membrane Proteins / metabolism
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Microfilament Proteins / genetics
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Microfilament Proteins / metabolism
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Microvilli / genetics
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Microvilli / metabolism
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Neoplasm Metastasis
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Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / genetics*
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Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / metabolism
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Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / pathology*
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Protein Kinase Inhibitors / pharmacology
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Protein Transport / drug effects
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RNA, Small Interfering / pharmacology
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Receptors, CCR / drug effects*
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Staurosporine / pharmacology
Substances
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CC chemokine receptor 9
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CCL25 protein, human
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Cadherins
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Chemokines, CC
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Cytoskeletal Proteins
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Membrane Proteins
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Microfilament Proteins
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Protein Kinase Inhibitors
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RNA, Small Interfering
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Receptors, CCR
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ezrin
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moesin
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radixin
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Staurosporine