Abstract
Objective:
To study the mechanism of baicalin on the cytokines of Th1/Th2 in murine model of asthma.
Methods:
The murine model of asthma was induced by OVA. Different doses of baicalin were orally administered to the mice respectively. The spleen cells were cultured 3 days for the measurement of IFN-gamma, IL-4, IL-5 and IL-10 by ELISA. After 2 days of culture, the spleen cells were treated with Trizol for extraction of total RNA. The gene expressions of T-bet, GATA-3 and STAT-6 were analyzed by RT-PCR.
Results:
The treatment with baicalin obviously decreased the production of IL-4 and IL-5 and the gene expression of GATA-3, STAT-6, but increased the production of IL-10.
Conclusion:
Baicalin may modulate the Th1/Th2 balance mainly by altering the gene expressions of GATA-3 and STAT-6 in vivo and increasing the production of IL-10.
Publication types
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English Abstract
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Asthma / chemically induced
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Asthma / immunology
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Asthma / pathology*
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Cells, Cultured
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Cytokines / immunology*
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Disease Models, Animal
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Female
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Flavonoids / administration & dosage
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Flavonoids / pharmacology*
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GATA3 Transcription Factor / metabolism
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Gene Expression Regulation / drug effects
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Lymphocytes / immunology
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Lymphocytes / metabolism
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Male
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Mice
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Mice, Inbred BALB C
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Ovalbumin / administration & dosage
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Random Allocation
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Reverse Transcriptase Polymerase Chain Reaction
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STAT6 Transcription Factor / metabolism
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Spleen / cytology
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Spleen / immunology
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Th1 Cells / drug effects*
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Th1 Cells / immunology
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Th1 Cells / metabolism
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Th2 Cells / drug effects*
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Th2 Cells / immunology
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Th2 Cells / metabolism
Substances
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Cytokines
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Flavonoids
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GATA3 Transcription Factor
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Gata3 protein, mouse
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STAT6 Transcription Factor
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Stat6 protein, mouse
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baicalin
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Ovalbumin