Objective: To study the effect of phytohormones on the regeneration of safflower, in order to provide a foundation for the rapid propagation of safflower seeds and genetic transformation.
Methods: The experiment used the cotyledons as the explant and studied the influence of calli induction, differentiation and rooting by phytohormones.
Results: 1. Different hormones by single using induced calli, but didn't directly produce adventitious buds; 2. With the combination of different phytohormones on the differentiation of adventitious buds were significantly different, the roots were also significantly different; 3. Cotyledon calli induction medium: MS + 3% Sucrose + 0.7% Agar + 1.0 mg/L NAA; differentiation medium: MS + 3% Sucrose + 0.7% Agar + 0.2 mg/L NAA + 1.0 mg/L 6-BA; rooting medium: 1/4 MS + 2.0 mg/L NAA + 0.5 mg/L IAA.
Conclusion: We have screened out the best culture medium of calli induction, differentiation and rooting via using singularity phytohormone and two phytohormones.