Cleavable linker for photo-cross-linked small-molecule affinity matrix

Bioconjug Chem. 2010 Jan;21(1):182-6. doi: 10.1021/bc900316q.

Abstract

The introduction of a cleavable site in a photoactivatable linker, which is used to immobilize small molecules on an affinity matrix via a site-nonselective carbene addition/insertion reaction, makes it possible to verify the presence of the immobilized small molecule on the affinity matrix. It also permits the efficient detection of proteins covalently bound to the immobilized small molecule.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Blotting, Western
  • Chromatography, Liquid
  • Cross-Linking Reagents / chemistry*
  • Disulfides / chemistry
  • Enzymes, Immobilized / chemistry
  • Enzymes, Immobilized / metabolism
  • Ligands
  • Mass Spectrometry
  • Methane / analogs & derivatives*
  • Methane / chemistry
  • Microspheres
  • Organophosphorus Compounds / chemistry
  • Photoaffinity Labels / chemistry*
  • Photochemistry*
  • Protein Binding
  • Protein Phosphatase 2 / chemistry
  • Protein Phosphatase 2 / metabolism
  • Sepharose / chemistry

Substances

  • Cross-Linking Reagents
  • Disulfides
  • Enzymes, Immobilized
  • Ligands
  • Organophosphorus Compounds
  • Photoaffinity Labels
  • carbene
  • Sepharose
  • Protein Phosphatase 2
  • Methane