Abstract
The stress-activated kinase Hog1p mediates arsenic tolerance by decreasing arsenite influx through the aquaglyceroporin Fps1p in Saccharomyces cerevisiae. Unexpectedly, we found that overexpression of FPS1 increased arsenite tolerance suggesting a physiological role of Fps1p in arsenic detoxification. Consistently, during arsenite treatment transcription of FPS1 gene was strongly upregulated, while Fps1p was not degraded and remained localized to the plasma membrane. Moreover, deletion of FPS1 gene resulted in arsenate sensitivity. Finally, transport experiments revealed that Fps1p in concert with the arsenite transporter Acr3p mediates arsenite efflux.
Copyright 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ATP-Binding Cassette Transporters / genetics
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ATP-Binding Cassette Transporters / metabolism
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Aquaglyceroporins / genetics
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Aquaglyceroporins / metabolism
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Arsenic / pharmacology
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Arsenites / metabolism*
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Biological Transport
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Blotting, Western
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Gene Expression Regulation, Fungal / drug effects
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Membrane Proteins / genetics
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Membrane Proteins / metabolism*
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Membrane Transport Proteins / genetics
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Membrane Transport Proteins / metabolism
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Microscopy, Fluorescence
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Mitogen-Activated Protein Kinases / genetics
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Mitogen-Activated Protein Kinases / metabolism
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Mutation
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Reverse Transcriptase Polymerase Chain Reaction
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Saccharomyces cerevisiae / drug effects
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism*
Substances
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ACR3 protein, S cerevisiae
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ATP-Binding Cassette Transporters
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Aquaglyceroporins
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Arsenites
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FPS1 protein, S cerevisiae
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Membrane Proteins
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Membrane Transport Proteins
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Saccharomyces cerevisiae Proteins
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YCF1 protein, S cerevisiae
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Green Fluorescent Proteins
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HOG1 protein, S cerevisiae
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Mitogen-Activated Protein Kinases
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arsenite
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Arsenic