Abstract
Carabrol, isolated from Carpesium macrocephalum, showed anti-inflammatory potential in LPS-induced RAW 264.7 murine macrophages. In present study, carabrol demonstrated the inhibitory activity on pro-inflammatory cytokines such as IL-1beta, IL-6 and TNF-alpha. In addition, mRNA and protein levels of iNOS and COX-2 were reduced by carabrol. Molecular analysis revealed that these suppressive effects were correlated with the inactivation of p38 and JNK via inhibition of NF-kappaB activation. Immunoblotting showed that carabrol suppressed LPS-induced degradation of I-kappaBalpha and decreased nuclear translocation of p65. Taken together, these results suggest that carabrol can be a modulator of pro-inflammatory signal transduction pathway in RAW 264.7 cells.
Copyright 2009 Elsevier Inc. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Active Transport, Cell Nucleus / drug effects
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Animals
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Anti-Inflammatory Agents, Non-Steroidal / chemistry
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Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
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Asteraceae / chemistry*
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Cell Line
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Cyclooxygenase 2 / biosynthesis
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Cyclooxygenase 2 / drug effects*
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I-kappa B Kinase / metabolism
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Interleukin-1beta / antagonists & inhibitors
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Interleukin-6 / antagonists & inhibitors
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JNK Mitogen-Activated Protein Kinases / antagonists & inhibitors
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JNK Mitogen-Activated Protein Kinases / metabolism
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Lipopolysaccharides / pharmacology
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Mice
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NF-kappa B / antagonists & inhibitors
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NF-kappa B / metabolism
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Nitric Oxide Synthase Type II / antagonists & inhibitors*
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Nitric Oxide Synthase Type II / biosynthesis
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Phosphorylation / drug effects
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Sesquiterpenes / chemistry*
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Sesquiterpenes / pharmacology
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Tumor Necrosis Factor-alpha / antagonists & inhibitors
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p38 Mitogen-Activated Protein Kinases / antagonists & inhibitors
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p38 Mitogen-Activated Protein Kinases / metabolism
Substances
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Anti-Inflammatory Agents, Non-Steroidal
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Interleukin-1beta
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Interleukin-6
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Lipopolysaccharides
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NF-kappa B
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Sesquiterpenes
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Tumor Necrosis Factor-alpha
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carabrol
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Nitric Oxide Synthase Type II
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Nos2 protein, mouse
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Ptgs2 protein, mouse
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Cyclooxygenase 2
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I-kappa B Kinase
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JNK Mitogen-Activated Protein Kinases
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p38 Mitogen-Activated Protein Kinases