Label-free dual assay of DNA sequences and potassium ions using an aptamer probe and a molecular light switch complex

Chem Commun (Camb). 2009 Dec 21:(47):7419-21. doi: 10.1039/b915994k. Epub 2009 Oct 22.

Abstract

A homogeneous assay is reported using Ru(phen)(2)(dppz)(2+) and a K(+)-binding aptamer for the selective and sensitive detection of a target oligonucleotide and potassium ions, based on reduction in fluorescence emission according to the formation of the G-quadruplex structure from the aptamer in the presence of K(+).

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aptamers, Nucleotide / analysis*
  • Aptamers, Nucleotide / chemistry
  • Cations / chemistry
  • G-Quadruplexes
  • Potassium / chemistry*
  • Sequence Analysis, DNA / methods*
  • Spectrometry, Fluorescence / methods*

Substances

  • Aptamers, Nucleotide
  • Cations
  • Potassium